Movie 5

Macrophages remove emerging PE cells in the absence of a heartbeat. Movie of an epi:GFP; mpeg1:mcherry embryo microinjected with a tnnt2 morpholino. One optical section is shown, after digitally fixing the extruded cell in the field of view. White arrowhead indicates extruding proepicardial (PE) cell (green) and magenta arrowhead shows a macrophage (red), which removes the PE cell by phagocytosis. Scale bar: 10 μm. Shown is a representative movie from ≥ 3 biological and ≥ 2 technical replicates.