Movie 5

Macrophages remove emerging PE cells in the absence of a heartbeat. Movie of an epi:GFP; mpeg1:mcherry embryo microinjected with a tnnt2 morpholino. One optical section is shown, after digitally fixing the extruded cell in the field of view. White arrowhead indicates extruding proepicardial (PE) cell (green) and magenta arrowhead shows a macrophage (red), which removes the PE cell by phagocytosis. Scale bar: 10 μm. Shown is a representative movie from ≥ 3 biological and ≥ 2 technical replicates.

Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells

Laura Andrés-Delgado, Alexander Ernst, María Galardi-Castilla, David Bazaga, Marina Peralta, Juliane Münch, Juan M. González-Rosa, Inês Marques, Federico Tessadori, José Luis de la Pompa, Julien Vermot, and Nadia Mercader

Development 2019. 146:None-None; doi: 10.1242/dev.174961