Example of Pull & Release experiment showing the deformation of a junction. The video was acquired at a frame rate of 16.4 frames / second, and it is reproduced at 16 frames / second. Some material inside the cells can be seen to follow the movement of the optical tweezer. We believe that is material consist of a vesicular organelle (possibly several smaller organelles) trapped by the laser and that this is pushed against the junction leading to its deformation. Scale bar is 5 um.