Movie 1

Three-dimensional view of an adult Drosophila melanogaster female scanned by μ-CT. The fly is separated along the XZ (2-14 s) and YZ axes (18-30 s) to reveal internal organs. Individual segmented organs are highlighted as follows: ocelli (red, 39 s); brain (blue, 40 s); lamina (yellow, 41 s); retina (red, 42 s); ventral nerve cord (blue, 43 s); esophagus & foregut (green, 44 s); midgut (yellow, 45 s); hindgut (red, 46 s); boli (purple, 47 s); Malpighian tubules (green, 48 s); crop (lavender; 49 s); salivary glands (turquoise, 50 s); early stage egg chamber (germarium-stage 6, blue, 51 s); mid stage egg chamber (stage 7-11, purple, 52 s); late stage egg chamber (stage 12-14; orange, 53 s); gastrulating embryo (purple, 54 s); heart (blue, 55 s); dorsal longitudinal muscles (orange, 56 s); dorsal ventral muscles (yellow, 57 s; green, 58 s; blue-green, 60 s); jump muscle (orange, 59 s); additional thorax and head muscles (61 s). Stain: 0.1N iodine; slow scan at an image pixel size of 1.25 μm.

Micro-computed tomography as a platform for exploring Drosophila development

Todd A. Schoborg, Samantha L. Smith, Lauren N. Smith, H. Douglas Morris, and Nasser M. Rusan

Development 2019. 146:None-None; doi: 10.1242/dev.176685