Movie 4

μ-CT of a wildtype (yw) pupa at stage P11. The pupa is separated along the YZ axis (5-9 s), followed by clipping along the XY axis from the anterior perspective (15 s). The brain is rendered as a 3D wireframe to reveal its position within the pupal case (18 s). Stain: 0.1N iodine; slow scan at an image pixel size of 1.4 μm.