Movie 6

RNAi depletion of spc105r leads to loss of head structures and pupal lethality, as revealed by μ-CT. Pupa is initially viewed from the dorsal perspective, anterior is to the right. Position of the ventral nerve cord (VNC, red) and brain (blue) within the thorax is revealed at 10s, the ‘head remnant’ (HR) structure containing an esophagus and labellelum are rendered as a yellow surface. Note orthogonal attachment of brain to VNC within the thorax. Stain: 0.1N iodine; slow scan at an image pixel size of 1.4 μm.