Movie 6

Sequential fusions of sibling cells derived from col1a2+ MPCs. col1a2Kaede; ubi:Zebrabow embryos were injured at 48 hpf, and imaged from 32 to 52 hpi. Representative snapshots and image quantification are shown in Figures 6E and S6, respectively. The green channel (col1a2Kaede), the red channel (ubi:Zebrabow) and the merged images are shown with time stamps indicated in the hh:mm format. Note that Kaede+ MPCs showed almost no RFP expression compared to muscle cells. A Kaede+ cell (white arrows) divided between 00:55 and 01:00. The anterior daughter cell (cyan arrows) fused with a muscle fiber between 11:10 and 11:15, while the posterior daughter cell (yellow arrows) fused with the same muscle fiber between 15:55 and 16:00. Concentrated Kaede expression in the nucleus after cell fusion was clearly visible (arrowheads of corresponding colors). A total of 31 fusion events were observed in movies of 12 embryos. Scale bar: 50 μm.

Single cell dynamics of embryonic muscle progenitor cells in zebrafish

Priyanka Sharma, Tyler D. Ruel, Katrinka M. Kocha, Shan Liao, and Peng Huang

Development 2019. 146:None-None; doi: 10.1242/dev.178400