Movie 10

Live-imaging of RNAP2 Ser2ph and H3K27ac in a single nucleus at the 256-cell stage in the presence of 10 μM JQ-1.
An embryo was injected with Fabs specific to RNAP2 Ser2ph (Alexa488), H3K27ac-Fab (Cy3), H3K9ac (Cy5) and soaked in 10 μM JQ-1, before and during imaging using a confocal microscope (FV1000). Twenty z-sections with 1 μm intervals were collected, and single sections were selected to make a movie for the 256-cell stage. Elapsed time is indicated as min:sec.