Movie 11

Live-imaging of RNAP2 Ser2ph and H3K27ac in a single nucleus under the presence of 10 μM JQ-1 at 512-cell stage.
An embryo was injected with Fabs specific to RNAP2 Ser2ph (Alexa488), H3K27ac-Fab (Cy3), and H3K9ac (Cy5) and soaked in 10 μM JQ-1, before and during imaging using a confocal microscope (FV1000). Twenty z-sections with 1 μm intervals were collected, and single sections were selected to make a movie for the 512-cell stage. Elapsed time is indicated as min:sec.

Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis

Yuko Sato, Lennart Hilbert, Haruka Oda, Yinan Wan, John M. Heddleston, Teng-Leong Chew, Vasily Zaburdaev, Philipp Keller, Timothee Lionnet, Nadine Vastenhouw, and Hiroshi Kimura

Development 2019. 146:None-None; doi: 10.1242/dev.179127