Movie 4
Live-imaging of RNAP2 Ser2ph and H3K27ac in a single nucleus at the 64-cell stage.
An embryo was injected with Fabs specific to RNAP2 Ser2ph (Alexa488), H3K27ac (Cy3), and H3K9ac (Cy5) were imaged using a confocal microscope (FV1000). Sixteen z-sections with 1 μm intervals were collected, and single sections for RNAP2 Ser2ph and H3K27ac were selected to make a movie for the 64-cell stage. Elapsed time is indicated as min:sec.
Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis
Development 2019. 146:None-None; doi: 10.1242/dev.179127