Movie 5

Live-imaging of RNAP2 Ser2ph and H3K27ac in a single nucleus at the 128-cell stage.
An embryo was injected with Fabs specific to RNAP2 Ser2ph (Alexa488), H3K27ac (Cy3), and H3K9ac (Cy5) were imaged using a confocal microscope (FV1000). Fifteen z-sections with 1 μm intervals were collected, and single sections for RNAP2 Ser2ph and H3K27ac were selected to make a movie for the 128-cell stage. Elapsed time is indicated as min:sec.