Movie 9

Live-imaging of RNAP2 Ser2ph, H3K27ac, and miR-430 transcripts in a single nucleus at the 512-cell stage in the presence of α-amanitin.
An embryo was injected with Fabs specific for RNAP2 Ser2ph (Cy3) and H3K27ac (Alexa488), and miR-430 Morpholino (Cy5), and then with α-amanitin, before imaging using a confocal microscope (FV1000). Twenty z-sections with 1 μm intervals were collected, and single sections were selected to make a movie for the 512-cell stage. Elapsed time is indicated as min:sec.

Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis

Yuko Sato, Lennart Hilbert, Haruka Oda, Yinan Wan, John M. Heddleston, Teng-Leong Chew, Vasily Zaburdaev, Philipp Keller, Timothee Lionnet, Nadine Vastenhouw, and Hiroshi Kimura

Development 2019. 146:None-None; doi: 10.1242/dev.179127