Movie 9

Live-imaging of RNAP2 Ser2ph, H3K27ac, and miR-430 transcripts in a single nucleus at the 512-cell stage in the presence of α-amanitin.
An embryo was injected with Fabs specific for RNAP2 Ser2ph (Cy3) and H3K27ac (Alexa488), and miR-430 Morpholino (Cy5), and then with α-amanitin, before imaging using a confocal microscope (FV1000). Twenty z-sections with 1 μm intervals were collected, and single sections were selected to make a movie for the 512-cell stage. Elapsed time is indicated as min:sec.