Movie 1

In vitro motility assay of wild-type or mutant NMY-2_HMM phosphorylated by LET-502(1-469)
Time-lapse images of Rhodamine-phalloidin-labelled F-actin filaments in motility chambers coated with NMY-2(wild-type)_HMM, NMY-2(S251A)_HMM or NMY-2(R252A)_HMM in the absence (top row) or presence of LET-502(1-469). Images were taken every 4 seconds. Frame rate 20 fps, timestamps min:sec. Scale bar, 10 μm.