Movie 5

A minimal amount of myosin is required for equatorial deformation in embryos substantially depleted of NMY-2
Time-lapse images of the central plane (1st and 3rd columns) and cell cortex (2nd and 4th columns) of dividing one-cell embryos expressing NMY-2::mCherry^sen (1st and 2nd columns) and LifeAct::GFP (3rd and 4th columns). Conditions are indicated for each row. Cortical images are maximum projections of seven z-sections 0.5 μm apart. First time point corresponds to anaphase onset (0:00) and frames are 5 seconds apart. Frame rate 4 fps, timestamps min:sec. Scale bar, 10 μm.