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Movie 1

MF-RNAscope permeates throughout the entire depth of myofibers.
Detection of Ache (green) and Myh2 (magenta) RNAs throughout a myofiber using MF- RNAs cope. Video is a confocal z-stack of the NMJ taken at 120x magnification; z-step distance between images = 0.5µm.