Caspase activation is observed before fragmentation of LECs in shits expressing LECs
Live imaging of the FRET ratio of the nuclear localized SCAT3 caspase activation indicator (ECFP/Venus) for shits expressing LECs under the restrictive temperature. The higher FRET ratio indicates higher caspase activity. Dorsal view. Anterior is up. Scale bar: 50 μm. Time interval: 100 sec.