Movie 2

The number of Clc::GFP puncta reduces as tissue remodeling progresses
Live imaging of endocytic activity in the LECs using Clc::GFP as a marker. Although GFP signal is uniformly distributed in the cytoplasm, the number of GFP puncta gradually decreases over time. Note that eliminated cells also have much stronger accumulation of GFP puncta, which is the post-process of cell elimination and therefore irrelevant to the analysis of this study. Dorsal view. Anterior is up. Scale bar: 10 μm. Time interval: 5 min.

Reduction of endocytic activity accelerates cell elimination during tissue remodeling of the Drosophila epidermal epithelium

Shinichiro Hoshika, Xiaofei Sun, Erina Kuranaga, and Daiki Umetsu

Development 2020. 147:None-None; doi: 10.1242/dev.179648