Movie 7

Live time-lapse confocal imaging of unc-40(hu226[unc-40::zf1]); mig-21(u787) dpy-19(e1314); huIs181[Pegl-17::zif-1-SL2-mcherry; Pmyo-2::mcherry] animals expressing plasma membrane and nuclear localized GFP in QL and the seam cells (transgene heIs63). Anterior is left. Images were acquired every 10 minutes for a total duration of 3 hours. Movies are played at 3 frames per second.