Movie 1

URX and BAG dendrites extend by stretch during embryo elongation. Related to Fig. 5.
Time-lapse movie of a wild-type embryo expressing egl-13pro:GFP, collected with dual-inverted selective plane illumination microscopy (diSPIM). BAG and URX extend short dendrites that contact the presumptive nose early in development and are then stretched to their full length during embryo elongation. The first eight frames were taken at 5 min intervals (40 min total, before the onset of embryo twitching), while the remaining frames were taken at 5 sec intervals to allow tracking of cells during rapid embryo movement. The movie is shown at a frame rate of 6 frames/sec. Selected frames have been annotated. Time stamp indicates minutes relative to start of movie. Approximate outline of embryo is drawn. Arrows and arrowheads indicate cell bodies and dendrite endings, respectively (BAG, blue; URX, green). The marker is also strongly expressed in an additional neuron (asterisk) and more weakly in elongated cells in the anterior.

Dendrites with specialized glial attachments develop by retrograde extension using SAX-7 and GRDN-1

Elizabeth R. Cebul, Ian G. McLachlan, and Maxwell G. Heiman

Development 2020. 147:None-None; doi: 10.1242/dev.180448