URX and BAG dendrite defects in grdn-1 mutants arise during embryo elongation. Related to Fig. 5.
Time-lapse movie of a grdn-1 mutant embryo expressing egl-13pro:GFP, collected with dual-inverted selective plane illumination microscopy (diSPIM). BAG and URX extend short dendrites that contact the presumptive nose early in development, but then detach from the nose and fail to extend. Frames were taken at 5-sec intervals, and the movie is shown at a frame rate of 6 frames/sec. Selected frames have been annotated. Time stamp indicates minutes relative to start of movie. Approximate outline of embryo is drawn. Arrows and arrowheads indicate cell bodies and dendrite endings, respectively (BAG, blue; URX, green). The marker is also strongly expressed in an additional neuron (asterisk) and more weakly in elongated cells in the anterior.