Movie 4

Live imaging of migrating cortical interneurons in an E14.5 cTKO brain slice at the midcortical position. Ventrolateral left, dorsomedial right. Movie Clip 1. Interneurons in cTKO brain slices begin to migrate in organized streams, but as time progresses, exit streams and infiltrate the cortical plate by the end of the 18-hour imaging period. Movie Clip 2. During the first 6 hours of imaging, some interneurons begin to leave the SVZ (Cell 1), whereas the majority of cells remain in the SVZ (Cells 2, 3). Movie Clip 3. During hours 6-12 of imaging, even more cells begin to evacuate the SVZ (Cells 5,6). Movie Clip 4. During the last 6 hours of imaging, migrating interneurons adopt more convoluted trajectories and infiltrate the cortical plate (Cells 7, 8, 9).