Movie 10

Time-lapse imaging of the Notch activity reporter Tg[tp1-MmHbb:kaede]^um15;Tg[kdrl:ras-Cherry]^s916 in a future arterial ISV, imaged from 29 hpf (right after conversion of the Kaede^green protein into Kaede^red) to 48,5 hpf. The Notch activity reporter is shown in kaede (red: old signal after conversion/green: new signal), all ECs are labeled in red). The tip of the growing secondary sprout is indicated by arrows. The tp1 signal (Notch activity) builds up before connection of the secondary sprout. (frame interval: 30 minutes). Scale bars, 25μm