Movie 3
Time-lapse recording of a Tg[fli1a:EGFP]y1 embryo (labeling ECs in green) mosaically overexpressing a pT2-zN1aICD-basfli-mCherry construct (orange-red) between 31 and 43hpf (frame interval: 6 minutes) showing formation of a transient perfused three-way connection between a wild-type secondary sprout and a NICDMOE primary ISV. Scale bar, 15μm