Movie 4
Time-lapse imaging of a Tg[fli1a:EGFP]y1;Tg[fli1a:B4GalT-mCherry]bns9 embryo (ECs depicted in grey, endothelial Golgi apparatus in red) showing EC polarity in an arterial ISV from 32 to 51 hpf. Arrows (green in primary ISV, blue in secondary sprout) point from the centre of the nucleus to the centre of the Golgi complex (frame interval: 15 minutes). Scale bars, 25μm