Movie 5

Time-lapse imaging of a Tg[fli1a:EGFP]^y1;Tg[fli1a:B4GalT-mCherry]^bns9 embryo (ECs depicted in grey, endothelial Golgi apparatus in red) showing EC polarity in a venous ISV from 33 to 55 hpf. Arrows (green in primary ISV, blue in secondary sprout) point from the centre of the nucleus to the centre of the Golgi complex (frame interval: 15 minutes). Scale bars, 25μm