Cytokinesis in the intestine epithelia
E8-E16 intestinal cell division in embryos expressing AIR-2::GFP (green, left, PH::mCherry in magenta), NMY-2::GFP (green, middle, tubulin::mCherry in magenta) or ZEN-4::GFP (green, right, tubulin::mCherry in magenta). The midbodies (indicated by arrowheads) form and move toward the apical surface. ZEN-4::GFP and NMY-2::GFP rapidly disappear, while AIR-2::GFP persists at the apical midline. Images are maximum Z projections of 10 planes spaced 1μm apart, taken every 60 seconds. Playback rate is 6 frames/second.
Xiaofei Bai, Michael Melesse, Christopher G. Sorensen Turpin, Dillon E. Sloan, Chin-Yi Chen, Wen-Cheng Wang, Po-Yi Lee, James R. Simmons, Benjamin Nebenfuehr, Diana Mitchell, Lindsey R. Klebanow, Nicholas Mattson, Eric Betzig, Bi-Chang Chen, Dhanya Cheerambathur, and Joshua N. Bembenek
Development
2020. 147:None-None; doi: 10.1242/dev.181099