Movie 6

Microtubule dynamics during E8-E16 in Aurora B mutants.
WT (top row) and air-2(or207) (bottom row) mutant embryos expressing TBB-1::GFP (green in merge, right movie) and PH::mCherry and H2B::mCherry (middle, magenta in merge). Spindle midzone microtubules in WT embryos move to the midline where microtubules accumulate. In Aurora B mutant embryos, spindle midzone microtubules are diminished and cells that fail cytokinesis (right pair of gut cells) delay microtubule accumulation compared with the cells that do not fail. Images are maximum Z projections of 10-15 z planes 1 μm apart that were acquired every 60 seconds. Playback rate is 6 frames/second.