3D movie of Wnt5a lineage in Wnt5a-/- E13.5 embryos
Wnt5a-/- embryos in Wnt5a-CreER; Rosa26td-Tomato/+ background were treated with a single dose of tamoxifen at E7.5 to label specifically Wnt5a expressing cells at this stage. Embryos were then collected to generate 3D reconstructed movie at E13.5. Color labels for different structures are the same as in Fig. 4J-J”.