Movie 5

Similar to Movie S3, for an EB differentiated in an elongated micropool (width=200um). In this case, Bra-GFP expression onsets from two different loci (around t.p. 121), at the two sides compressed against the well walls. At t.p. 130 the EB pops out of the channel, resulting in a change in its focus.

Prediction and control of symmetry breaking in embryoid bodies by environment and signal integration

Naor Sagy, Shaked Slovin, Maya Allalouf, Maayan Pour, Gaya Savyon, Jonathan Boxman, and Iftach Nachman

Development 2019. 146:None-None; doi: 10.1242/dev.181917