Movie 1

Removal of LDs from MII oocytes.
A two-step centrifugation pushes aggregated LDs into the PV space, and the resultant LDs were easily aspirated using a micropipette. The first and third MII oocytes show typical examples of successful LD removal, whereas the second MII oocyte retained some LDs even after centrifugation (mp4; 1.0 MB).