Movie 1

Removal of LDs from MII oocytes.
A two-step centrifugation pushes aggregated LDs into the PV space, and the resultant LDs were easily aspirated using a micropipette. The first and third MII oocytes show typical examples of successful LD removal, whereas the second MII oocyte retained some LDs even after centrifugation (mp4; 1.0 MB).

Synthesis and maintenance of lipid droplets are essential for mouse preimplantation embryonic development

Ryutaro Aizawa, Megumi Ibayashi, Takayuki Tatsumi, Atsushi Yamamoto, Toshiaki Kokubo, Naoyuki Miyasaka, Ken Sato, Shuntaro Ikeda, Naojiro Minami, and Satoshi Tsukamoto

Development 2019. 146:None-None; doi: 10.1242/dev.181925