Live imaging for Δ50cyclin B2-Venus and mCherry-securin in the GDF9-Ccnb1−/− oocytes. The GDF9-Ccnb1−/− oocytes expressing Δ50cyclin B2-Venus (Green) and mCherry-securin (Cherry-red) with time shown (h:min:s) relative to the release from IBMX. Bar, 20 μm.