Movie 3

Fast confocal imaging reveals changes in neural crest cell filopodial dynamics after AQP-1 manipulation. Projected images from spinning disk time-lapse microscopy of migrating lead neural crest cells in whole embryo culture electroporated with either (top) pMES control, (middle) AQP-1 FL or (bottom) AQP-1 Morpholino (MO). Each movie sequence shows the cell membrane label (Gap43-mTurquoise2) to highlight the cell protrusion dynamics. Images were collected in 30 second intervals and shown here for approximately 11 min.