Fast confocal imaging reveals changes in neural crest cell filopodial dynamics after AQP-1 manipulation. Projected images from spinning disk time-lapse microscopy of migrating lead neural crest cells in whole embryo culture electroporated with either (top) pMES control, (middle) AQP-1 FL or (bottom) AQP-1 Morpholino (MO). Each movie sequence shows the cell membrane label (Gap43-mTurquoise2) to highlight the cell protrusion dynamics. Images were collected in 30 second intervals and shown here for approximately 11 min.
Rebecca McLennan, Mary C. McKinney, Jessica M. Teddy, Jason A. Morrison, Jennifer C. Kasemeier-Kulesa, Dennis A. Ridenour, Craig A. Manthe, Rasa Giniunaite, Martin Robinson, Ruth E. Baker, Philip K. Maini, and Paul M. Kulesa
Development
2020. 147:None-None; doi: 10.1242/dev.185231