Movie 3

Fast confocal imaging reveals changes in neural crest cell filopodial dynamics after AQP-1 manipulation. Projected images from spinning disk time-lapse microscopy of migrating lead neural crest cells in whole embryo culture electroporated with either (top) pMES control, (middle) AQP-1 FL or (bottom) AQP-1 Morpholino (MO). Each movie sequence shows the cell membrane label (Gap43-mTurquoise2) to highlight the cell protrusion dynamics. Images were collected in 30 second intervals and shown here for approximately 11 min.

Neural crest cells bulldoze through the microenvironment using Aquaporin 1 to stabilize filopodia

Rebecca McLennan, Mary C. McKinney, Jessica M. Teddy, Jason A. Morrison, Jennifer C. Kasemeier-Kulesa, Dennis A. Ridenour, Craig A. Manthe, Rasa Giniunaite, Martin Robinson, Ruth E. Baker, Philip K. Maini, and Paul M. Kulesa

Development 2020. 147:None-None; doi: 10.1242/dev.185231