Movie 1
Migration of Co MO-injected NC cells. NC explants of embryos that were injected with 8 ng Co MO and 500 pg GAP43-GFP and 400 pg H2B-mcherry RNA to mark the membrane (green) and the nucleus (red). The cells were observed using spinning disc microscopy (40x objective). Z-stacks of 5-7 slices were taken every 90 seconds for a time period of 20 minutes. The cells exhibited intense protrusive activity.
The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled
Development 2020. 147:None-None; doi: 10.1242/dev.186338