Movie 2

Migration of Trio MO-injected NC cells. NC cells injected with 8 ng Trio MO and co-injected with 500 pg GAP43-GFP and 400 pg H2B-mcherry RNA to visualize the membrane (green) and the nucleus (red). The cells were observed using spinning disc microscopy (63x objective). Z-stacks of 5-7 slices were taken every 30 seconds for a time period of 20 minutes. The cells showed membrane blebbing without protrusion formation.

The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled

Marie-Claire Kratzer, Sarah F. S. Becker, Anita Grund, Anne Merks, Jakub Harnoš, Vítězslav Bryja, Klaudia Giehl, Jubin Kashef, and Annette Borchers

Development 2020. 147:None-None; doi: 10.1242/dev.186338