Movie 3
Migration of NC cells injected with Trio MO in combination with hTrio DNA. NC explants of embryos injected with 8 ng Trio MO and 10 pg hTrio DNA, 500 pg GAP43-GFP RNA and 400 pg H2B-mcherry RNA were co-injected to visualize the membrane (green) and the nucleus (red). The cells were observed using spinning disc microscopy (40x objective). Z-stacks of 5-7 slices were taken every 5 minutes for a time period of 20 minutes. The co-expression of Trio restored protrusion formation in Trio depleted cells.
The Rho guanine nucleotide exchange factor Trio is required for neural crest cell migration and interacts with Dishevelled
Development 2020. 147:None-None; doi: 10.1242/dev.186338