Movie 2

Short-term lineage (temporary)tracing of Otx2b-derived cells
Time-lapse imaging of otx2-positive cells in the otx2b:Venus-NLS reporter line. Embryos were dorsally mounted with the anterior end to the top and imaged from 26 hpf to 32hpf. Tissue sections covering about 40μm were chosen with a z interval of 1 μm. Images were acquired at 2:30 (min:sec) intervals. Maximum intensity projections of the fluorescent images merged with transmitted light images are shown. Perdurance of the Venus fluorescent protein in the hindbrain domain (marked with a yellow circle) can be seen. An image file highlighting the region of interest (circle) was inserted within the movie subsequently using iMovie (10.1.13).