Movie 4

Cell sorting at the MHB
Time-lapse imaging of wnt1+cells in the wnt1:Venus-NLS reporter line (supporting movie file for images shown in Fig.5A). Embryos were dorsally mounted with the anterior end to the top and imaged from 10.5 hpf to 12 hpf. Tissue sections covering about 30μm were chosen with a z interval of 1 μm. Images were acquired at 2:30 (min:sec) intervals. Maximum intensity projection of images are shown. The movement of a straggling wnt1-cell (arrow) and its active migration towards the group of other boundary cells is shown. An image file highlighting the region of interest (arrow) was inserted at the beginning of the movie frame using iMovie (10.1.13).