A sequence of optical sections (z-sections, z =1 μm interval, total 40 μm), acquired on the dorsal-ventral axis of the double transgenic fish, otx2b:HRAS- mKate2 and gbx1:Venus, is shown. These are un-injected control fish at 14hpf. An overlapping otx2 (magenta)-gbx1 (cyan) domain, spanning a few cell layers, can be observed.