Dynamics of maternal actb2 mRNAs tracked with dSmR3nd-mCherry protein and single antisense fluorescent probe.
One-cell stage embryos were injected with 1 ng dSmR3nd-mCherry protein and 300 pg fluorescein-labeled actb2 antisense P3 probe and imaged at the 4-cell stage. Dual-color dSmR3nd/antisense probe puncta represented endogenous actb2 mRNAs. The video was presented by a z-projection. Scale bar, 10 μm.