Dynamics of zygotic actb2 mRNAs tracked with dSmR3nd-mCherry protein and continuously expressed antisense probe.
One-cell stage embryos were injected with 1 ng in vitro purified dSmR3nd-mCherry protein and 20 pg pU6:actb2-1xP3;ef1α:GFP plasmid DNA and imaged at the shield stage. dSmR3nd-mCherry signal could be observed to move dynamically in the cytosol, which represented actb2 mRNA. The video was represented by a single z-plane. Scale bar, 10 μm.