Movie 4

3-D traction force analysis (+Blebbistatin). Time-lapse images of the initial branching process of eSMG cultures (E13) treated with 50 mM blebbistatin. The isolated epithelial rudiments were embedded in Matrigel-DMEM/F-12 mixture containing 5% fluorescent microbeads (2.0 mm diameter). The images were acquired for 7 h with 10-min intervals.