Movie 1

SEIP-1 expression pattern during ovulation
Ovulation imaged in the genome-edited animals expressing SEIP-1::mScarlet (magenta). arrows in left and right panels indicate SEIP-1::mScarlet expression in the fifth gonadal sheath cell until the oocyte completed the ovulation. Left panel indicates the mScarlet (magenta) channel only, middle channel indicates DIC only, right channel indicates the merged image of mScarlet and DIC. Images are z-max with 10 z planes taken every 30 seconds with 1 μm step size. Timing is indicated in lower right of the left panel. Playback rate is 2 frames/second. Scale bar is indicated in the left panel.