Movie 2

Time-lapse of stage 25 frontonasal mass dissected and cultured in ROCKi treated media. Nuclei were stained with Hoechst dye and imaged with confocal microscope, at 10X magnification (R1, ROCK inhibitor treatment organ culture). Tracks appear more disorganized and tissue failed to narrow in the mediolateral axis. The center of the frontonasal mass is relatively stationary in the center where cartilage will form at later stages, similar to control tissues.