High-resolution light-sheet microscopy time-lapse imaging of lung bud elongation
Time-lapse movie showing the development of an E11.5 left lung rudiment carrying the ShhGC/+; ROSAmT/mG construct. The specimen was mounted in a hollow cylinder made from low-melting-point agarose and filled with matrigel to replicate the native microenvironment and promote near-physiological growth. Imaging was done using the Zeiss Z.1 Lightsheet system for 34h. (AVI 2.38 MB).