Tok and Slit-N expression are non-overlapping. Z-stack video of the embryo from Fig. 2A-C of Tok::GFP labeled with anti-GFP (green) and anti-Slit-N (magenta). Note that Tok::GFP appeared in specific motor neurons, in motor axons (arrows), and in underlying cell bodies, while Slit primarily labeled underlying midline glia (arrowhead). Movie comprises 134 images, with each Z slice 211.2 μm thick collected using a Leica Tissue Imager wide-field microscope with Thunder deconvolution processing. Bar indicates 200μm.