Movie 2
Single plane time-lapse experiment visualising cytoplasmic SunTag-hb mRNA bound by MCP-RFP (magenta), and translation sites or mature proteins bound by scFv-NG (green). Embryos are from females carrying single copies of the scFv-NG and tdMCP-RFP transgenes crossed to SunTag-hb-MS2 transgenic males. Imaged with a 63x objective and a time resolution of ~ 57 msec per frame. Time stamp is in sec:msec.
Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo
Development 2021. 148:None-None; doi: 10.1242/dev.196121