Movie 4

Single plane time-lapse experiment showing a single protein being released from a translation site (green, scFv-NG) and diffusing away from the mRNA (magenta, tdMCP-mRuby3-CAAX) and site of translation. The embryo genotype and imaging settings are as described for movie 3, except the time resolution is ~ 1.7 sec per frame.

Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo

Daisy J. Vinter, Caroline Hoppe, Thomas G. Minchington, Catherine Sutcliffe, and Hilary L. Ashe

Development 2021. 148:None-None; doi: 10.1242/dev.196121