Movie 7.

Live imaging of microtubule dynamics and BB movements in a FP cell at the 5s stage. wt embryos were injected with EB3-GFP, Centrin-GFP (Fire LUT, coding for fluorescence intensity) and membrane-Cherry (green) mRNAs at the 16-cell stage. and then imaged for 8min every 10 seconds at the 5s stage. White arrow at t=0ʺ points at the BB. White arrows at t=150ʺ, t=160ʺ, t=280ʺ and t=290ʺ point at posterior membrane deformation and yellow arrowheads at t=150ʺ and t=280ʺ point at microtubules linking BB and posterior membrane at the spot where the membrane bends and toward which BB will move. Dorsal view. Corresponds to FigS2D.

Planar polarization of cilia in the zebrafish floor-plate involves Par3-mediated posterior localization of highly motile basal bodies

Antoine Donati, Isabelle Anselme, Sylvie Schneider-Maunoury, and Christine Vesque

Development 2021. 148:None-None; doi: 10.1242/dev.196386