Movie 1

Acute and reversible loss of PM localization of Dlg::GFP^KI and Lgl::mCherry under hypoxia in follicular cells
Ovaries from a 2-day old dlg::GFP^KI, lgl::mCherry female were dissected and imaged live in an environment-controlled micro chamber. Hypoxic (0.5% O₂) gas was flashed into the chamber at 0 minute to induce hypoxia and normal air was flashed into chamber at 111 minutes for reoxygenation. Time intervals are 3 minutes during hypoxia and 10 seconds during reoxygenation. Note the incomplete loss of Dlg::GFP from the PM at the end of hypoxia. Time stamp: hh:mm:ss.