Movie 2

3-D rotations of control and gadd45-RNAi embryonic renal tubules
Live in vivo imaging of control (left) and ctB>gadd45-RNAi (right) anterior renal tubules at embryonic stage 14, where tubule cell nuclei are labelled using ctB-gal4 driven expression of nRFP. 3-D rotations around the renal tubules in vivo illustrates the differences in morphology between control tubules and those lacking Gadd45; whilst no Cut positive tubule nuceli are visible outside of the control tubules (left panel), significant numbers of Cut positive nuclei are observed outside of the misshapen tubule lacking Gadd45 expression. Scale bar represents 20μm. See also Figure 2 and Figure S2.