Movie 3
Live-imaging of gadd45-RNAi renal tubule morphogenesis
Live in vivo imaging of ctB>gadd45-RNAi anterior renal tubules at embryonic stage 14, where
tubule cell nuclei are labelled using ctB-gal4 driven expression of nRFP. A RFP-positive tubule nucleus can be observed leaving the anterior tubule (arrowhead); many other RFP-positive punctae are observed moving within the extracellular space around the developing tubules which we envision to be tubule cell corpses contained within migratory hemocytes. Scale bar represents 20μm. See also Figure 2 and Figure S2.
Metabolically active and polyploid renal tissues rely on graded cytoprotection to drive developmental and homeostatic stress resilience
Development 2021. 148:None-None; doi: 10.1242/dev.197343